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Regulation of antigen processing and presentation by cysteine proteases in cancer

School of Medicine, Dentistry and Biomedical Sciences | PHD

Applications are now CLOSED
Funding
Funded
Reference Number
SMED-2231-1228
Application Deadline
None specified
Start Date
None specified

Overview

This project will examine the role of cysteine cathepsin S in MHC Class I and II antigen processing and presentation in a model of lung adenocarcinoma. Specifically, the project will consider antigen diversification, T Cell receptor (TCR) diversity and the resultant impact on the T cell repertoire. This project will involve significant in vivo modelling, various antigenic assays and multi panelled immunophenotyping via high parameter flow cytometry

In lung cancer, the role of cysteine cathepsin S in MHC Class I and II antigen processing and presentation and how resultant peptides present to CD4+ and CD8+ T cells has yet to be elucidated. Our current data suggests that loss of cathepsin S reduces lung tumour burden with concomitant reduction in pulmonary inflammation. Using in vivo models of lung cancer, various ex vivo co-culture assays, this project will examine MHC expression (both Class I and II) in the antigen presenting cells, evaluate antigenic diversity/repertoire, resultant T cell (sub)types and their functional status when cathepsin S expression is altered.
This project is suitable for students with a clear background in immunology and/or cancer biology and possess a strong interest in understanding how the immune system interacts with cancer and how such interactions can be exploited in the development of new therapies. The successful PhD candidate will join a cross-centre collaborative team and will receive training in a wide range of laboratory techniques including immune cell isolation/purification from tumour and lung tissue, cell culture, histological staining, microscopy, ELISAs, western blotting, mass spectrometry, and high parameter flow cytometry.

Funding Information

Project Summary
Supervisor

Dr Donna Small

More Information

askmhls@qub.ac.uk

Research Profile


Mode of Study

Full-time: 3 Years


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