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Enhancing Protein Thermal Stability for Melt Processing

School of Pharmacy | PHD
Funding
Unfunded
Reference Number
PMY/2251/SC2
Application Deadline
None specified
Start Date
None specified

Overview

Protein therapeutics are an important class of medicines with renowned efficacy in the treatment of several relevant disorders. Despite significant advantages, protein delivery remains a significant challenge. Melt processing is a versatile and attractive processing method that is solvent free, high throughput and can potentially yield high encapsulation efficiencies for protein therapeutics. However, compatibility of proteins with this process is often hampered due loss of protein activity due to high heat and shear forces. Improving protein stability during melt processing can provide an innovative approach to formulating proteins for efficient delivery.

The proposed PhD project will focus on engineering stable suitable melt processed encapsulated therapeutic proteins. Polymer-based strategies such as PEGylation have been widely used to increase thermal stability and inhibit aggregation in solution. The scientific approach of this project is to exploit rational protein-polymer design and improve protein stability during the melt-extrusion process. To do so, the impact of melt processing on protein stability will be investigated. Protein stability will be safeguarded via covalent and non-covalent polymer-based strategies (e.g., PEGylation) and structural and functional stability examined. Protein-polymer conjugates will be characterized and biologically activity evaluated in vitro and in vivo. The successful candidate will be part of a highly interdisciplinary project and have the opportunity to learn about protein formulation, bioconjugation, biological testing and continuous manufacturing.

Training will be provided in a range of techniques including protein characterization, bioconjugation, hot-melt extrusion technology and performance characterization including in vitro bioactivity and stability assays. The student will also develop generic research skills in scientific writing, literature reviewing, time management and delivery of presentations, nationally and internationally.

The PhD student will be encouraged to engage in a variety of impact activities, disseminate the research project findings through publications, public talks, and participate in QUB showcase events.

Project Summary
Supervisor

Dr Sheiliza Carmali

More Information

s.carmali@qub.ac.uk

Research Profile


Mode of Study

Full-time: 3 Years


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