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Wastewater monitoring for SARS-CoV-2 and other microbial pathogens

School of Biological Sciences | PHD

Applications are now CLOSED
Funding
Funded
Reference Number
SBIO-2020-1249
Application Deadline
24 March 2023
Start Date
1 October 2023

Overview

As SARS-CoV-2 is shed in high levels in faeces, wastewater testing has become a complementary, early-warning strategy for outbreaks of Covid-19, aiding governmental decision making around future infection prevention and control policies. The Queen’s University led Northern Ireland SARS-CoV-2 wastewater surveillance programme currently monitors wastewater treatment sites across NI for SARS-CoV-2 and its variants. This project seeks to extend our research beyond SARS-CoV-2 to investigate whether other pathogens can similarly be monitored within wastewater and determine what the clinical implications of such surveillance might be.

Wastewater surveillance provides a cheap, non-invasive method to determine the level of infection in a large community within a single sample (composite 24-hour sewage samples represent an aggregate of an entire population within the sewerage network). Importantly, as faecal shedding occurs in the pre-symptomatic phase, evidence also suggests that it can be used as an early warning system to indicate when levels of SARS-CoV-2 are rising (or falling) in the community and before individuals require hospitalisation. Currently the NI SARS-CoV-2 wastewater programme monitors wastewater from across the province for SARS-CoV-2 and its variants using RT-qPCR, ddPCR and sequencing / bioinformatics based approaches. In this studentship we will extend our work beyond SARS-CoV-2 to investigate if other pathogens (e.g. respiratory syncytial virus, influenza, enterovirus D68, etc.) can similarly be detected in wastewater and if their monitoring can be used to inform clinical decision making. The aims of this are project are: 1) Further develop methods for pathogen detection and analysis within wastewater; 2) Using those methods and sequencing approaches developed in (1) investigate the prevalence of selected pathogens within wastewater, correlating pathogen load with clinical case numbers; and 3) Develop modelling tools to link pathogen levels in wastewater with clinical cases, to be used as a tool to predict infection rates within the community.

The studentship will provide training in state-of-the-art molecular microbiology techniques, with the successful student joining a team of academics from across Queen’s University Belfast, Department of Health, NI Water Ltd and the Public Health Agency focused on wastewater based epidemiology.

Specific skills/experience required: Background in microbiology, biochemistry, pharmacy, biomedical science, or related discipline.

Start Date: 1 October 2023

Duration: 3 years

How to apply: Applications must be submitted online via: https://dap.qub.ac.uk/portal/user/u_login.php

Funding Information

Applicants for this and a number of other projects will be in competition for studentships funded by the Northern Ireland Department for the Economy (DfE).
Candidates must be normally resident in the UK for the three year period prior to 1 September 2023. For non-EU nationals, the main purpose of residence must not have been to receive full-time education. Non-UK or Irish nationals must also have pre-settled or settled status (EU nationals) or settled status (non-EU nationals).
Full eligibility criteria: https://www.economy-ni.gov.uk/publications/student-finance-postgraduate-studentships-terms-and-conditions

Project Summary
Supervisor

Professor John McGrath

More Information

askmhls@qub.ac.uk

Research Profile


Mode of Study

Full-time: 3 years


Funding Body
DfE
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