Location and time:  WWIEM-Basement Seminar Room -10am-11am 

Lecturer : Dr. Ileana Micu

• Microscopy basics (Thursday 20^th February 2020)        

                -Light, wavelength, resolution

                -Component of a microscope

                -Objectives

                -Bright field microscopy, phase contrast, DIC

                -Applications

                -Image formation in microscopy and image analysis

• Fluorescence (Thursday 27^th February 2020)   

                 -Fluorescence and Jablonski diagram

                 -Light sources, filters, dichroic

                 -Dyes for fluorescence microscopy

                 -Wide field fluorescence microscopy

                 -Calcium imaging

• Confocal microscopy (Friday  6^th March 2020)            

                 -Confocal laser scanning microscopy concept

                  -Detectors (PMT, Hybrid detectors)

                  -Photo bleaching, photo destruction

                  -Spectral imaging; FRET, FRAP

                  -3D imaging

•     Advanced optical microscopy techniques (to be resckedule)

                  -Two-photon excitation microscopy

                  - Second harmonic generation (SHG)

                  -Coherent Anti-Stokes RAMAN Microscopy (C.A.R.S.)

                  -Super-resolution microscopy (TIRF, SIM, STED, STORM)

                  -Light sheet microscopy

• Electron microscopy (to be announced)

               -Transmission electron microscopy

               -Sample preparation